Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples

Autor:	Giovanna S. Rocha, Martin K. L. Silva, Ivana Cesarino
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	reduced graphene oxide enterotoxin A Staphylococcus aureus impedimetric immunosensor Technology Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85
Zdroj:	Materials, Vol 13, Iss 7, p 1751 (2020)
Druh dokumentu:	article
ISSN:	1996-1944
DOI:	10.3390/ma13071751
Popis:	A simple, cheap, and less aggressive immobilization procedure for biomolecules using reduced graphene oxide (rGO) was employed to prepare an impedimetric immunosensor for detection of staphylococcal enterotoxin A (SEA) from Staphylococcus aureus in milk samples. The scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy (EIS) were used to monitor the single steps of the electrode assembly process. The glassy carbon (GC)/rGO platform detected the antigen-antibody binding procedures of SEA with concentrations of 0.5 to 3.5 mg L−1 via impedance changes in a low frequency range. The impedimetric immunosensor was successfully applied for the determination of SEA in milk samples.
Databáze:	Directory of Open Access Journals
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