| Autor: |
Yinyin Zhou, Kunna Liu, Xingyou Li, Yanpin Qin, Yuehuan Zhang, Yang Zhang, Zhiming Xiang, Haitao Ma, Jun Li, Ziniu Yu |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Aquaculture Reports, Vol 18, Iss , Pp 100541- (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2352-5134 |
| DOI: |
10.1016/j.aqrep.2020.100541 |
| Popis: |
Beclin-1 plays a central role in autophagy, which is well documented in mammals. However, the identity and function of Beclin-1 remain largely unclear in mollusks. In this study, a Beclin-1 gene (ChBeclin-1) was identified and characterized from Crassostrea hongkongensis. The full length of ChBeclin-1 cDNA encoded a predicted 442-amino acid protein with a deduced molecular weight of 51.11 kDa and a theoretical isoelectric point of 4.71. The putative amino acid sequence of ChBeclin-1 contained a typical Bcl-2 homology domain 3 (BH3), a coiled-coil domain (CCD), and an Atg6 domain. Phylogenetic analysis showed that ChBeclin-1 was clustered with Beclin-1 from other mollusks such as Crassostrea gigas, Crassostrea virginica, and Mizuhopecten yessoensis. ChBeclin-1 mRNA was constitutively expressed in all detected tissues, with the highest relative expression in the gills. Additionally, significant inductions in the mRNA level of ChBeclin-1 were observed after bacterial stimulations, indicating its involvement in the innate immune response. The knockdown of ChBeclin-1 via RNA interference impeded the formation of autophagosomes, denoting ChBeclin-1 plays a role in autophagy. Moreover, in subcellular localization and dual-luciferase reporter assays, ChBeclin-1 protein was found to be localized mainly in the endoplasmic reticulum, and its overexpression suppressed the transcriptional activity of an NF-κB reporter gene in a dose-dependent manner in HEK 293T cells. Collectively, these findings provide experimental evidence of a functional Beclin-1 in autophagy and demonstrate its involvement in the innate immunity of C. hongkongensis. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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