Autor: |
Parul Mehrotra, Najmuddin Saquib, Kanury Rao |
Jazyk: |
angličtina |
Rok vydání: |
2015 |
Předmět: |
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Zdroj: |
Bio-Protocol, Vol 5, Iss 8 (2015) |
Druh dokumentu: |
article |
ISSN: |
2331-8325 |
DOI: |
10.21769/BioProtoc.1447 |
Popis: |
Fluctuations in metabolite levels in mammalian cells are the most direct form of readout of the cellular metabolic state. The current protocol describes a method for pulse labeling and subsequent isolation of metabolites from adherent mammalian cells. The isolated metabolites can be identified and quantified by mass-spectrometry, allowing for estimation of the rates of synthesis and removal of metabolites from the system being analyzed. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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