| Autor: |
Parul Mehrotra, Najmuddin Saquib, Kanury Rao |
| Jazyk: |
angličtina |
| Rok vydání: |
2015 |
| Předmět: |
|
| Zdroj: |
Bio-Protocol, Vol 5, Iss 8 (2015) |
| Druh dokumentu: |
article |
| ISSN: |
2331-8325 |
| DOI: |
10.21769/BioProtoc.1447 |
| Popis: |
Fluctuations in metabolite levels in mammalian cells are the most direct form of readout of the cellular metabolic state. The current protocol describes a method for pulse labeling and subsequent isolation of metabolites from adherent mammalian cells. The isolated metabolites can be identified and quantified by mass-spectrometry, allowing for estimation of the rates of synthesis and removal of metabolites from the system being analyzed. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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