Prediction of the minimum amount of anti-adhesive agent required for entire intra-abdominal cavity using fluorescent dye
| Autor: | Ji-Hyeon Park, A Reum Park, Kiwon Kim, Seo Hyun Shin, Youngbae Jeon, Woon Kee Lee, Donghyuk Lee, Jeong-Heum Baek |
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| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Korean Journal of Clinical Oncology, Vol 20, Iss 1, Pp 18-26 (2024) |
| Druh dokumentu: | article |
| ISSN: | 1738-8082 2288-4084 |
| DOI: | 10.14216/kjco.24004 |
| Popis: | Purpose Studies on the appropriate amount of anti-adhesive agents for preventing postoperative adhesion are lacking. This animal study aimed to investigate the distribution of an anti-adhesive agent in the abdominal cavity and estimate the necessary amount to cover the entire cavity. Methods Fluorescent dye Flamma-552 was conjugated to Guardix-sol to create Guardix-Flamma, which was laparoscopically applied to the abdominal cavity of two 10-kg pigs in different amounts: 15 mL for G1 and 35 mL for G2. After 24 hours, the distribution of Guardix-Flamma was examined under the near-infrared mode of the laparoscope, and the thickness was measured in tissues from the omentum, small, and large intestine by immunohistochemistry. Results The average area of the abdominal cavity in 10 kg pigs was 2,755 cm2. Guardix-Flamma fluorescence was detected in the greater omentum, ascites in the pelvis, and right quadrant area in G1, whereas in G2, it was detected everywhere. On average, the total thickness of G1 and G2 were 12.68±9.80 μm and 18.16±15.57 μm, respectively. Guardix-Flamma thickness applied to the omentum, small, and large intestines of G2 were 1.31-, 1.45-, and 1.49-times thicker than those of G1, respectively, and were all statistically significant (P |
| Databáze: | Directory of Open Access Journals |
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