| Autor: |
Mitsuaki Ishihara, Takeshi Kujiraoka, Tadao Iwasaki, Makoto Nagano, Mayumi Takano, Jun Ishii, Masahiro Tsuji, Hajime Ide, Irina P. Miller, Norman E. Miller, Hiroaki Hattori |
| Jazyk: |
angličtina |
| Rok vydání: |
2005 |
| Předmět: |
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| Zdroj: |
Journal of Lipid Research, Vol 46, Iss 9, Pp 2015-2022 (2005) |
| Druh dokumentu: |
article |
| ISSN: |
0022-2275 |
| DOI: |
10.1194/jlr.D500018-JLR200 |
| Popis: |
Apolipoprotein A-V (apoA-V) is a recently discovered apolipoprotein that appears to have a role in plasma triglyceride (TG) transport. We have developed an ELISA for apoA-V using monoclonal antibodies that has a lower limit of detection of 0.3 ng/ml and linearity up to 20 ng/ml. The ELISA was then used to quantify plasma apoA-V in 196 healthy subjects and 106 patients with insulin-resistant diabetes mellitus. In the healthy subjects, total apoA-V concentration was 179.2 ± 74.8 ng/ml, and it was greater in females than in males (P < 0.005). It was correlated positively with the plasma HDL cholesterol (r = 0.32, P < 0.0001), apoA-I (r = 0.27, P = 0.0001), and apoE (r = 0.18, P = 0.011) concentrations and negatively with plasma TG concentration (r = −0.22, P = 0.021). In relation to single nucleotide polymorphism 3 (−1131C/T) of the apoA-V gene, apoA-V concentration was higher in the T/T type than in the C/C type (P < 0.01). Plasma TG concentration was lower in the T/T type than in the C/C or C/T type (P < 0.05).ApoA-V concentration was lower in the diabetic patients (69.4 ± 44.3 ng/ml; P < 0.01) than in the healthy controls. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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