Determination of ABO blood grouping from dentine and pulp by absorption-elution technique

Autor: Abhishek Singh Nayyar, K V Chalapathi, G Kartheek, K Pramod, Abhishek Ranjan Pati, K Indirapriyadarsini, Dushyantsinh Vala
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Advances in Human Biology, Vol 8, Iss 2, Pp 102-111 (2018)
Druh dokumentu: article
ISSN: 2321-8568
2348-4691
DOI: 10.4103/AIHB.AIHB_59_17
Popis: Introduction: Blood grouping has been one of the cornerstones of identification of biological material. Mostly, teeth and bones are the only significant tissues remaining in mass disasters such as aircraft crash or bomb blasts and hence used in human identification. It has also been suggested that blood group antigens in the pulp and dentine are preserved even up to 2 years after death of an individual. Aim: In the present study, an attempt was made to determine the ABO blood grouping from the dentine and pulp by absorption-elution (AE) technique. Materials and Methods: The study group included 60 patients requiring extraction due to periodontal or orthodontic purposes. Extraction procedure was carried out under local anaesthesia following an aseptic protocol. After extraction, the socket blood was collected for blood group determination which served as control for the study. The blood grouping was performed by AE technique using powdered dentine and dental pulp. Statistical Analysis: The statistical analysis was carried out using the Statistical Package for the Social Sciences version 19, SPSS Inc., Chicago, USA. The statistical analysis for comparison of teeth component with ABO blood groups with the age period and gender differentiation was done using Chi-square test. P < 0.05 was considered as significant. Results: Out of 60 samples tested for ABO blood grouping, dentine and pulp showed no significant difference with age and gender; results were more positive in the age group in which individuals were
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