The Rapid Antigen Detection Test for SARS-CoV-2 Underestimates the Identification of COVID-19 Positive Cases and Compromises the Diagnosis of the SARS-CoV-2 (K417N/T, E484K, and N501Y) Variants

Autor: Carlos Barrera-Avalos, Roberto Luraschi, Eva Vallejos-Vidal, Andrea Mella-Torres, Felipe Hernández, Maximiliano Figueroa, Claudia Rioseco, Daniel Valdés, Mónica Imarai, Claudio Acuña-Castillo, Felipe E. Reyes-López, Ana María Sandino
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Frontiers in Public Health, Vol 9 (2022)
Druh dokumentu: article
ISSN: 2296-2565
DOI: 10.3389/fpubh.2021.780801
Popis: Timely detection of severe acute respiratory syndrome due to coronavirus 2 (SARS-CoV-2) by reverse transcription quantitative polymerase chain reaction (RT-qPCR) has been the gold- strategy for identifying positive cases during the current pandemic. However, faster and less expensive methodologies are also applied for the massive diagnosis of COVID-19. In this way, the rapid antigen test (RAT) is widely used. However, it is necessary to evaluate its detection efficiency considering the current pandemic context with the circulation of new viral variants. In this study, we evaluated the sensitivity and specificity of RAT (SD BIOSENSOR, South Korea), widely used for testing and SARS-CoV-2 diagnosis in Santiago of Chile. The RAT showed a 90% (amplification range of 20 ≤ Cq 30. In SARS-CoV-2 variant detection, RAT had a 42.8% detection sensitivity in samples with RT-qPCR amplification range 20 ≤ Cq
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