Histological study of fresh versus frozen semitendinous muscle tendon allografts

Autor: Alexandre Carneiro Bitar, Luiz Augusto Ubirajara Santos, Alberto Tesconi Croci, João Alberto Ramos Maradei Pereira, Edgard N. França Bisneto, Arlete Mazzini Miranda Giovani, Claudia Regina G. C. M. Oliveira
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Zdroj: Clinics, Vol 65, Iss 3, Pp 297-303 (2010)
Druh dokumentu: article
ISSN: 1807-5932
1980-5322
DOI: 10.1590/S1807-59322010000300010
Popis: OBJECTIVE: The purpose of this study was to histologically analyze allografts from cadaveric semitendinous muscle after cryopreservation at -80°C in comparison to a control group kept at only -4°C to test the hypothesis that the histological characteristics of the tissue are maintained when the tendons are kept at lower temperatures. METHODS: In a tissue bank, 10 semitendinous tendons from 10 cadavers were frozen at -80ºC as a storage method for tissue preservation. They were kept frozen for 40 days, and then a histological study was carried out. Another 10 tendon samples were analyzed while still "fresh". RESULTS: There was no histological difference between the fresh and frozen samples in relation to seven variables. CONCLUSIONS: Semitendinous muscle tendon allografts can be submitted to cryopreservation at -80ºC without suffering histological modifications.
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