Comparative study on effect of different embryo culture media on in vitro blastocyst production in goats
| Autor: | PUJA GOEL, A K GOEL, A K BHATIA, S D KHARCHE |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Indian Journal of Animal Sciences, Vol 86, Iss 10 (2016) |
| Druh dokumentu: | article |
| ISSN: | 0367-8318 2394-3327 |
| DOI: | 10.56093/ijans.v86i10.62325 |
| Popis: | The present study was aimed to assess the efficacy of different culture media for blastocyst development. Oocytes (2,539) from 1,059 ovaries recovered by follicle puncture technique were utilized for present investigation. In experiment 1, effect of different embryo culture media (Gr. 1; mKSOMaa; Gr. 2; TCM-199+OEC; Gr. 3; mSOFaa and Gr. 4; mCR2aa) and in experiment 2, effect of sequential embryo culture medium, Gr. 1: mKSOMaa containing 5% FBS (1–3 days) and 10% FBS (4–10 days); Gr. 2: 0.8% BSA (1–3 days) and continuous embryo culture medium, 10% FBS (4–10 days); Gr. 3: 0.8% BSA (1–10 days) and Gr. 4: 10% FBS (1–10 days) on in vitro blastocyst development potential was evaluated. Significant higher (P |
| Databáze: | Directory of Open Access Journals |
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