| Autor: |
Hongming Chen, Longshan Zhao, Guangjing Li, Donglei Leng, Panqin Ma, Lijin Tong, Tianhong Zhang |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
|
| Zdroj: |
Asian Journal of Pharmaceutical Sciences, Vol 9, Iss 5, Pp 286-292 (2014) |
| Druh dokumentu: |
article |
| ISSN: |
1818-0876 |
| DOI: |
10.1016/j.ajps.2014.07.008 |
| Popis: |
In order to evaluate the pharmacokinetic profile of paliperidone extended-release tablets in vivo, a simple and rapid ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method was developed and validated for the determination of paliperidone in beagle dog plasma. Paliperidone and diazepam (internal standard) were extracted from plasma samples with diethyl ether, and then separated on a C18 column (2.1 × 50 mm, 2.6 μm) under gradient elution with methanol–0.1% formic acid at a flow rate of 0.3 ml/min. The compounds were detected using a triple-quadrupole mass spectrometer equipped with an electrospray ionization (ESI) source. The validated method was linear over the concentration range of 1.00–1000.00 ng/ml and the lower limit of quantitation was 1.00 ng/ml. The intra-day and inter-day precision values were not more than 15% (relative standard deviation |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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