Four-dimensional NOE-NOE spectroscopy of SARS-CoV-2 Main Protease to facilitate resonance assignment and structural analysis

Autor: A. J. Robertson, J. Ying, A. Bax
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Magnetic Resonance, Vol 2, Pp 129-138 (2021)
Druh dokumentu: article
ISSN: 2699-0016
DOI: 10.5194/mr-2-129-2021
Popis: Resonance assignment and structural studies of larger proteins by nuclear magnetic resonance (NMR) can be challenging when exchange broadening, multiple stable conformations, and 1H back-exchange of the fully deuterated chain pose problems. These difficulties arise for the SARS-CoV-2 Main Protease, a homodimer of 2 × 306 residues. We demonstrate that the combination of four-dimensional (4D) TROSY-NOESY-TROSY spectroscopy and 4D NOESY-NOESY-TROSY spectroscopy provides an effective tool for delineating the 1H–1H dipolar relaxation network. In combination with detailed structural information obtained from prior X-ray crystallography work, such data are particularly useful for extending and validating resonance assignments as well as for probing structural features.
Databáze: Directory of Open Access Journals