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Objective: This study’s aim was conducted to reveal the relationship between the attitudes of the Nursing Department students towards gender roles and their attitudes towards lesbian and gay individuals. Materials and Methods: 1079 students studying in the 1st, 2nd, 3rd and 4th grade at related University Faculty of Health Sciences Nursing Department in the spring semester of the 2018-2019 academic year constituted the population of the study. In the study, no sample selection was made and it was studied with 606 nursing students (Power=99.0%). The data were collected using the Socio-Demographic Data Form, the Attitudes toward Lesbians and Gay Men Scale-Short Version(ATLG-R), and the Gender Role Attitude Scale(GRAS). Results: It was determined that 90.3% of the participants knew the concept of gender roles. When the students were asked whether they would discriminate against LGBTs individual due to their sexual orientation/identity, 69.6% of them stated that they would not discriminate, 15% of them stated that they were undecided, and 14.8% of them stated that they would discriminate. It was concluded that the mean score of the GRAS of the participating students was 65.044±14.622, and the ATLG-R scale score were 30.533±8.454. It was found that there was a significant negative relationship between the ATLG-R scale scores and the GRAS scores (p |