USE OF FOURIER TRANSFORM INFRARED (FTIR) SPECTROSCOPY TO PREDICT VFA AND AMMONIA FROM IN VITRO RUMEN FERMENTATION
| Autor: | Franco Tagliapietra, Giulia Rossi, Stefano Schiavon, Alessandro Ferragina, Claudio Cipolat-Gotet, Giovanni Bittante |
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| Jazyk: | English<br />Croatian |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Poljoprivreda, Vol 21, Iss 1 sup, Pp 130-134 (2015) |
| Druh dokumentu: | article |
| ISSN: | 1330-7142 1848-8080 |
| DOI: | 10.18047/poljo.21.1.sup.30 |
| Popis: | The aim of the present study was to develop a FTIR method to quantify amounts and proportions of volatile fatty acids (VFA) and ammonia nitrogen (N-NH3) in fermentation fluids collected in vitro using innovative Bayesian models as chemometric technique. A set of 170 fluids, collected before and after 4 in vitro incubations of 8 diets in 5 replication plus 5 blanks, were analysed for VFA, N-NH3 and scanned using the MilcoScan FT2 (Foss Electric, Hillerød, Denmark) in the spectral range between 5000 and 900 cm-1. A Bayes B model was used to calibrate equations for each fermentative trait. The calibration equation predicts well VFA and N-NH3 amounts in calibration and also in validation (R2 VAL ranged from 0.93 to 0.83 for isovaleric and n-butyric acid, respectively). However, the prediction of VFA expressed as proportions of total amount was much less accurate (R2 VAL ranged from 0.81 to 0.52 for iso-valeric and n-butyric acid, respectively). In conclusion, FTIR and Bayesian models can be used as tools to accurately predict VFA amounts in vitro. |
| Databáze: | Directory of Open Access Journals |
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