Phytochemical and Anti-Inflammatory Analysis of Prunus africana Bark Extract

Autor: Gitonga Godfrey Mutuma, Joseph Ngeranwa, Machocho Alex Kin g’ori, Silas Kiruki
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Research Journal of Pharmacognosy, Vol 7, Iss 4, Pp 31-38 (2020)
Druh dokumentu: article
ISSN: 2345-4458
2345-5977
DOI: 10.22127/rjp.2020.229941.1583
Popis: Background and objectives: Inflammation is associated with various diseases; Prunus africana (Hook f.) is commonly used in Meru community of Kenya in management of inflammation. Medicinal plants contain phytochemicals associated with pharmacological activities; so, the aim of the present study was evaluating the anti-inflammatory activity of Prunus africana bark extract and qualitative analysis phytochemical of its phytochemicals. Methods: Five hundred mg of the powdered P. africana stem bark was extracted using 1.5 liters of dichloromethane for 24 h. The anti- inflammatory activity was evaluated against carrageenan paw induced edema in mice. The ability of the extracts to suppress the paw inflammation was expressed as a percentage inhibition of paw edema in five groups each comprising of five mice. Group I was treated with DMSO, group II with diclofenac (100 mg/Kg) and experimental groups III, IV and V with 50, 100 and 150 mg/Kg of the plant extract. The ability of the extracts to suppress the paw inflammation was expressed as a percentage inhibition of paw edema in mice. The qualitative phytochemical analysis was conducted using the standard protocols. Results: The percentages paw edema inhibition after the 4th h in the positive control and the experimental groups I, II and III were 13.61, 32.85, 25.15 and 5.92%, respectively. The qualitative evaluation of stem bark extract illustrated presence of tannins, saponins, flavonoids, alkaloids, quinones, cardiac glycosides, terpenoids, phenolics and coumarins. Conclusion: Dichloromethane stem bark extract of the P. africana presented anti-inflammatory activity hence a possible candidate for extraction of active anti-inflammatory compounds.
Databáze: Directory of Open Access Journals
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