Production of intravenous human dengue immunoglobulin from Brazilian-blood donors
| Autor: | Frederico Leite Gouveia, Renata Mendes Batista de Oliveira, Antônio Edson de Souza Lucena, Marli Tenório Cordeiro, Ana Cristina Lima Leite |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Brazilian Journal of Pharmaceutical Sciences, Vol 49, Iss 4, Pp 821-829 (2013) |
| Druh dokumentu: | article |
| ISSN: | 2175-9790 1984-8250 |
| DOI: | 10.1590/S1984-82502013000400022 |
| Popis: | Dengue represents an important health problem in Brazil and therefore there is a great need to develop a vaccine or treatment. The neutralization of the dengue virus by a specific antibody can potentially be applied to therapy. The present paper describes, for the first time, the preparation of Immunoglobulin specific for the dengue virus (anti-DENV IgG), collected from screened Brazilian blood-donations. Production was performed using the classic Cohn-Oncley process with minor modifications. The anti-DENV IgG was biochemically and biophysically characterized and fulfilled the requirements defined by the European Pharmacopoeia. The finished product was able to neutralize different virus serotypes (DENV-1, DENV-2, and DENV-3), while a commercial IgG collected from American blood donations was found to have low anti-dengue antibody titers. Overall, this anti-DENV IgG represents an important step in the study of the therapeutic potential and safety of a specific antibody that neutralizes the dengue virus in humans. |
| Databáze: | Directory of Open Access Journals |
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