Development of Algorithm for Identification of Brucellosis Agent Cultures Using MALDI-TOF Mass-Spectrometry
| Autor: | D. V. Ul’Shina, D. A. Kovalev, O. V. Bobrysheva, G. I. Lyamkin, A. A. Khudoleev, Yu. V. Siritsa, A. N. Kulichenko |
|---|---|
| Jazyk: | ruština |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Проблемы особо опасных инфекций, Vol 0, Iss 4, Pp 96-99 (2015) |
| Druh dokumentu: | article |
| ISSN: | 0370-1069 2658-719X |
| DOI: | 10.21055/0370-1069-2015-4-96-99 |
| Popis: | Given are the results of direct protein profiling of brucella strain collection using matrix laser desorption/ionization time-of-flight mass-spectrometry (MALDI-TOF MS). Standardized is the method for brucellosis agent culture disinfection and subsequent assessment. Obtained are 59 representative protein profiles of six major species ( B. melitensis, B. abortus, B. canis, B. ovis, B. neotomae ), which are included into electronic database of mass-spectra, integrated in Biotyper v 3.0 software (Bruker Daltonics, Germany). Analysis of mass-spectra of brucella strains under investigation revealed 17 genus-specific fragments within the mass range of 2000-20000 Da, the combination of which can be used for Brucella spp . identification. In addition, described are the fragments, specific for certain brucella species and strains, promising as markers for intraspecific differentiation. Based on the data received developed is a standardized algorithm of identification and differentiation of brucellosis agent cultures applying MALDI-TOF mass spectrometry. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|