| Autor: |
Mégane Da Mota, Julien Cau, Julio Mateos-Langerak, Armelle Lengronne, Philippe Pasero, Jérôme Poli |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
STAR Protocols, Vol 2, Iss 2, Pp 100525- (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1667 |
| DOI: |
10.1016/j.xpro.2021.100525 |
| Popis: |
Summary: This protocol describes how to culture, image, and determine the nuclear position of a fluorescently tagged DNA locus in the 3D nucleoplasm of fixed Saccharomyces cerevisiae cells. Here, we propose a manual scoring method based on widefield images and an automated method based on 3D-SIM images. Yeast culture conditions have to be followed meticulously to get the best biological response in a given environment.For complete details on the use and execution of this protocol, please refer to Forey et al. (2020). |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
