Efficient determination of cysteine sulphoxides in Allium plants applying new biosensor and HPLC-MS² methods
| Autor: | K. Ziegert, W. Schütze, H. Schulz, M. Keusgen, F. Gun, E. R. J. Keller |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | Journal of Applied Botany and Food Quality, Vol 80, Iss 1 (2012) |
| Druh dokumentu: | article |
| ISSN: | 1613-9216 1439-040X |
| Popis: | Cysteine sulphoxide (CSO) contents of 16 different accessions of garlic (Allium sativum L.) and 15 varieties of onion (Allium cepa L.) were measured using two different rapid analytical methods: a biosensoric approach and a newly developed HPLC-MS2 technique. Both methods allow quantification of naturally occurring cysteine sulphoxides present in Allium plants without time-consuming sample pretreatment such as derivatisation of amino acid derivatives prior to HPLC-separation. It has been found that the amount of alliin, which is the predominant CSO occurring in garlic, varies between 0.2 and 2.2 g/100 g dry matter. Contrary to that, isoalliin representing the main CSO in onion has been detected in significantly lower amounts (0.3 to 1.25 g/100 g dry matter). |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|