Analysis of JAK2 V617F mutation in Tunisian patients with myeloproliferative neoplasms

Autor: Soumaya Chadi, Tarak Dhaouadi, Imen Sfar, Hela Baccouche, Rym Nabli, Neila Ben Romdhane, Taïeb Ben Abdallah, Yousr Gorgi
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: European Journal of Inflammation, Vol 19 (2021)
Druh dokumentu: article
ISSN: 2058-7392
20587392
DOI: 10.1177/20587392211006538
Popis: We aimed to investigate the prevalence of the JAK2 V617F mutation in Tunisian patients with myeloproliferative neoplasms (MPN) and to look for possible associations with diseases’ presentation. In this context, JAK2 V617F polymorphism was detected by PCR-RFLP and direct sequencing in 213 MPN patients (109 with polycythemia vera (PV), 93 with essential thrombocythemia (ET) and 11 with primary myelofibrosis (PMF)), 77 unclassified patients with thrombosis (UPT) and 95 healthy control subjects. The JAK2 V617F mutant allele was present by either PCR-RFLP or direct sequencing in 158 (74.17%) MPN patients while all UPT and controls were negative. Besides, the JAK2 V617F mutation was significantly more frequent in patients with PV 98 (89.9%) than in ET 54 (58.1%) and PMF 6 (54.5%) groups, p
Databáze: Directory of Open Access Journals
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