Age-related changes of choroid plexus morphology, vascularization and epithelial proliferation

Autor: Samardžija Golub D., Andrejić-Višnjić Bojana M., Đolai Matilda A., Ilić-Sabo Jelena R., Amidžić Jelena P., Nikin Zoran, Rakočević Natali Z., Aleksić Nikola N., Dolamić Borislav E., Vukomanović-Đurđević Biserka B.
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Archives of Biological Sciences, Vol 73, Iss 2, Pp 197-204 (2021)
Druh dokumentu: article
ISSN: 0354-4664
1821-4339
DOI: 10.2298/ABS201210014S
Popis: The choroid plexus (ChP) is essential for brain homeostasis by regulating the secretion of the cerebrospinal fluid (CSF). Despite a substantial body of work on the pathologically alterations of the ChP, there is lack of data concerning the naturally occurring morphological changes asso-ciated with ageing. In this study, we investigated 30 human ChP tissue samples that were divided into 3 groups according to age. Morphometric analysis of psammoma bodies (PBs) on hematoxylin and eosin (H&E)-stained samples and immunohistochemical analysis of ChP were performed (using antibodies to transmembrane phosphoglycoprotein protein CD34 and nuclear protein Ki-67). Amyloid deposits were detected using Congo red staining. Middle-aged and older individuals exhibited a significantly higher numerical density (ndPB) mostly as increased immature forms, which led us to question the proposed nomenclature. The proliferation rate of the ChP epithelium did not show significant difference between groups. The vascular area was markedly decreased and accompanied by amyloid deposition in blood vessel walls. While the deposits were limited to middle-sized blood vessels in the middle-aged group, in the older group deposits were also present around small vessels. The identified major morphological alterations of ageing ChP tissue provide further understanding of disfunctions of the blood-cerebrospinal fluid barrier that underlie neurodegenerative disorders.
Databáze: Directory of Open Access Journals
Externí odkaz: