Field evaluation of an iELISA and CF test for detection of IgG antibodies against Chlamydophila abortus in goats, sheep and rams
| Autor: | M. Trávníček, D. Kováčová, M.R. Bhide, P. Zubrický, L. Čisláková |
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| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: | |
| Zdroj: | Veterinární Medicína, Vol 47, Iss 7, Pp 195-198 (2002) |
| Druh dokumentu: | article |
| ISSN: | 0375-8427 1805-9392 |
| DOI: | 10.17221/5823-VETMED |
| Popis: | Blood sera samples from 99 clinically healthy goats, 230 sheep and 171 rams were investigated by CF test and indirect ELISA. In case of goats, 3.03% seroprevalence was detected, in sheep it was 3.04%, whereas, in case of rams seroprevalence was 0% by using complement fixation test. Using iELISA in the same groups the seroprevalences observed were, 24.24% in goats, 11.30% in sheep and 5.30% in rams. Indirect ELISA was found to be comparatively more sensitive than CF test in all three groups of animals for detection of IgG antibodies against Chlamydophila abortus. The iELISA used in this study can be used for screening at herd level like CF test, as there is moderate agreement (Kappa - 0.426) between these two tests. |
| Databáze: | Directory of Open Access Journals |
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