Culturing of kerationocytes in serum-free medium

Autor: Mohammad Shokerzadeh, Mahnaz Mahmoudi Rad, Mansoureh Mirzaei, Nariman Mosef, Maedeh Ahangari
Jazyk: English<br />Persian
Rok vydání: 2009
Předmět:
Zdroj: Journal of Mazandaran University of Medical Sciences, Vol 19, Iss 70, Pp 67-71 (2009)
Druh dokumentu: article
ISSN: 1735-9260
1735-9279
Popis: (Received 9 February, 2009 ; Accepted 6 May, 2009)AbstractBackground and purpose: To establish a simple method for preparation of pure epidermal cell in serum-free medium, without co culturing with lethally irradiated 3T3 cells.Materials and methods: A piece of skin biopsy was taken from a rat. After trypsinazation to separate epidermal layer, the epidermis was cut into explants 1×1 mm and were laid at 50 mL on tissue cultured flasks. Then, the explants were covered with serum-free keratinocyte growth medium, while the culture medium was changed every two days. The keratinocyte colonies were expanded in flasks and proliferated to 70% confluency on day 21. After two subcultures, the cells were frozen.Results: Pure epidermal cells are established by this technique in a serum-free keratinocyte growth medium, under feeder cell-free condition.Conclusion: Culturing of keratinocytes in serum-free medium is proved to be a useful and simple method for keratinocyte isolation. The successful culturing of keratinocytes from a small skin biopsy can be useful in the treatment of major burn wounds.J Mazand Univ Med Sci 2009; 19(70): 67-71 (Persian)
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