Direct sequencing of small genomes on the Pacific Biosciences RS without library preparation

Autor:	Paul Coupland, Tamir Chandra, Mike Quail, Wolf Reik, Harold Swerdlow
Jazyk:	angličtina
Rok vydání:	2012
Předmět:	Pacific Biosciences PacBio next-generation DNA Sequencing single molecule real time library prep Biology (General) QH301-705.5
Zdroj:	BioTechniques, Vol 53, Iss 6, Pp 365-372 (2012)
Druh dokumentu:	article
ISSN:	1940-9818 0736-6205
DOI:	10.2144/000113962
Popis:	We have developed a sequencing method on the Pacific Biosciences RS sequencer (the PacBio) for small DNA molecules that avoids the need for a standard library preparation. To date this approach has been applied toward sequencing single-stranded and double-stranded viral genomes, bacterial plasmids, plasmid vector models for DNA-modification analysis, and linear DNA fragments covering an entire bacterial genome. Using direct sequencing it is possible to generate sequence data from as little as 1 ng of DNA, offering a significant advantage over current protocols which typically require 400–500 ng of sheared DNA for the library preparation.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/b215e4d024064e8298a2f38818ba0098 Zobrazit plný text záznamu View record in DOAJ