In vitro testing of immunosupressive effects of mesenchymal stromal cells on lymphocytes stimulated with alloantigens
| Autor: | Daniel Lysak, Tomas Vlas, Monika Holubova, Michaela Miklikova, Pavel Jindra |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Biomedical Papers, Vol 159, Iss 2, Pp 215-219 (2015) |
| Druh dokumentu: | article |
| ISSN: | 1213-8118 1804-7521 |
| DOI: | 10.5507/bp.2013.072 |
| Popis: | Aims: Mesenchymal stromal cells (MSC) derived from adult bone marrow or adipose tissue offer the potential to open a new frontier in medicine. MSC are involved in modulating immune response and tissue repair in vitro and in vivo. Experimental evidence and preliminary clinical studies have demonstrated that MSC exhibit an important immunomodulatory function in patients with graft versus host disease (GVHD) following allogeneic hematopoietic stem cell transplantation. The immunosuppressive properties of MSC have already been exploited in the clinical setting. However the precise mechanisms are being still investigated. Methods: We examined the immunosuppressive function of MSC by coculturing them with stimulated HLA incompatible allogeneic lymphocytes in a mixed lymphocyte culture test. The metabolic and proliferative activity of lymphocytes was determined by MTT test. Results: After stimulation with alloantigens the presence of MSC caused significant decrease of absorbance levels by 62% (P |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|