| Autor: |
Ria Lassaunière, Charlotta Polacek, Gregers J. Gram, Anders Frische, Jeanette Linnea Tingstedt, Maren Krüger, Brigitte G. Dorner, Anthony Cook, Renita Brown, Tatyana Orekov, Tammy Putmon-Taylor, Tracey-Ann Campbell, Jack Greenhouse, Laurent Pessaint, Hanne Andersen, Mark G. Lewis, Anders Fomsgaard |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
npj Vaccines, Vol 6, Iss 1, Pp 1-13 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2059-0105 |
| DOI: |
10.1038/s41541-021-00419-z |
| Popis: |
Abstract New generation plasmid DNA vaccines may be a safe, fast and simple emergency vaccine platform for preparedness against emerging viral pathogens. Applying platform optimization strategies, we tested the pre-clinical immunogenicity and protective effect of a candidate DNA plasmid vaccine specific for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The DNA vaccine induced spike-specific binding IgG and neutralizing antibodies in mice, rabbits, and rhesus macaques together with robust Th1 dominant cellular responses in small animals. Intradermal and intramuscular needle-free administration of the DNA vaccine yielded comparable immune responses. In a vaccination-challenge study of rhesus macaques, the vaccine demonstrated protection from viral replication in the lungs following intranasal and intratracheal inoculation with SARS-CoV-2. In conclusion, the candidate plasmid DNA vaccine encoding the SARS-CoV-2 spike protein is immunogenic in different models and confers protection against lung infection in nonhuman primates. Further evaluation of this DNA vaccine candidate in clinical trials is warranted. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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