| Autor: |
Sanaa A. Rizk, Fateheya M. Metwally, Asmaa M. Elfiky, Asmaa A. Mahmoud, Nadia A. Badawi, Nevin E. Sharaf, Mahmoud M. Elhefnawi |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
|
| Zdroj: |
Journal of Genetic Engineering and Biotechnology, Vol 16, Iss 1, Pp 53-56 (2018) |
| Druh dokumentu: |
article |
| ISSN: |
1687-157X |
| DOI: |
10.1016/j.jgeb.2018.02.003 |
| Popis: |
Altered miRNAs were associated with cigarette smoking. The study aimed to examine the gene expression level of plasma let-7a among healthy smokers and compared it with the non-smokers. Forty subjects were recruited for the present study and classified into 21 smokers and 19 non-smokers, age, and sex were matched. The software that used to design functional primers was MIRprimer. Quantitative real-time PCR was employed to compare the relative expression of plasma let-7a. Results showed that the level of let-7a was down-regulated in smokers to 0.34fold (p = 0.006) that of the non-smokers. Plasma let-7a showed an area under curve (AUC) of 0.749 with sensitivity 43% and specificity 100%. In conclusion, plasma let-7a was significantly down-regulated in the smokers, and it might be considered a candidate biomarker to discriminate between smokers and non-smokers. Keywords: Plasma let7-a, Biomarker, Cigarette smoking, Software MIRprimer |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
