| Autor: |
Daniel Klevebring, Mårten Neiman, Simon Sundling, Louise Eriksson, Eva Darai Ramqvist, Fuat Celebioglu, Kamila Czene, Per Hall, Lars Egevad, Henrik Grönberg, Johan Lindberg |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
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| Zdroj: |
PLoS ONE, Vol 9, Iss 8, p e104417 (2014) |
| Druh dokumentu: |
article |
| ISSN: |
1932-6203 |
| DOI: |
10.1371/journal.pone.0104417 |
| Popis: |
Accurate estimation of systemic tumor load from the blood of cancer patients has enormous potential. One avenue is to measure the presence of cell-free circulating tumor DNA in plasma. Various approaches have been investigated, predominantly covering hotspot mutations or customized, patient-specific assays. Therefore, we investigated the utility of using exome sequencing to monitor circulating tumor DNA levels through the detection of single nucleotide variants in plasma. Two technologies, claiming to offer efficient library preparation from nanogram levels of DNA, were evaluated. This allowed us to estimate the proportion of starting molecules measurable by sequence capture ( |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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