Staphylococcal cassette chromosome mec elements from Staphylococcus intermedius group (SIG) isolates from dogs in a center for veterinary diagnostics in Brazil

Autor: Regina Ruiz, Valcilaine Teixeira Barbosa, Teresa de Lisieux Guedes Ferreira Lôbo, Rafael Aleixo dos Santos Silva, Rodrigo José Nunes Calumby, Suzanne Pinheiro Vieira, Diogo Brandão, Felipe Mendes Zorzi, Rossana Teotônio de Farias Moreira, Valter Alvino, Ticiano Gomes do Nascimento, Luciano Aparecido Meireles Grilo, Camila Braga Dornelas, Elsa Masae Mamizuka, Lara Mendes de Almeida
Jazyk: English<br />Portuguese
Rok vydání: 2021
Předmět:
Zdroj: Ciência Rural, Vol 51, Iss 9 (2021)
Druh dokumentu: article
ISSN: 1678-4596
0103-8478
DOI: 10.1590/0103-8478cr20200936
Popis: ABSTRACT: Methicillin resistance in the Staphylococcus intermedius group (SIG) has emerged in small animal practice. Methicillin-resistant SIG (MRSIG) members have been implicated as causes of infections in both companion animals and humans. Staphylococcal cassette chromosome mec (SCCmec) elements carry the mecA/C genes, which encode for the transpeptidase PBP2a (PBP2’) responsible for β-lactam antibiotic resistance in staphylococci. This study examined the SCCmec types of MRSIG isolates from different clinical specimens of dogs that exhibited methicillin MIC ≥ 0.5 μg/mL by an automated identification and susceptibility system in a Center for Veterinary Diagnostics in São Paulo, Brazil. Susceptibility to methicillin was determined by broth microdilution testing, and Oxoid® M.I.C.Evaluator® strips. PBP2a production was detected using a latex agglutination assay. SCCmec typing was performed according to the International Working Group on the Classification of Staphylococcal Cassette Chromosome Elements (IWG-SCC) guidelines. SCCmec type II (2A), SCCmec type III (3A), composite SCC structures consisting of a class A mec gene complex in addition to multiple ccr gene complexes, and non-typable SCCmec elements were reported in these MRSIG isolates. SCCmec type variants differing from those so far acknowledged by IWG-SCC were found, indicating new rearrangements in the genetic context of mecA in these canine MRSIG isolates.
Databáze: Directory of Open Access Journals
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