| Autor: |
Pan Ma, Fuchun Gong, Hanming Zhu, You Qian, Lingzhi He, Jiaoyun Xia, Zhong Cao |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Molecules, Vol 27, Iss 16, p 5164 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
1420-3049 |
| DOI: |
10.3390/molecules27165164 |
| Popis: |
Nitrite (NO2−) is a common pollutant and is widely present in the environment and in human bodies. The development of a rapid and accurate method for NO2− detection is always a very important task. Herein, we synthesized a partnered excited-state intermolecular proton transfer (ESIPT) fluorophore using the “multi-component one pot” method, and used this as a probe (ESIPT-F) for sensing NO2−. ESIPT-F exhibited bimodal emission in different solvents because of the solvent-mediated ESIPT reaction. The addition of NO2− caused an obvious change in colors and tautomeric fluorescence due to the graft of NO2− into the ESIPT-F molecules. From this basis, highly sensitive and selective analysis of NO2− was developed using tautomeric emission signaling, achieving sensitive detection of NO2− in the concentration range of 0~45 mM with a detection limit of 12.5 nM. More importantly, ESIPT-F showed the ability to anchor proteins and resulted in a recognition-driven “on-off” ESIPT process, enabling it to become a powerful tool for fluorescence imaging of proteins or protein-based subcellular organelles. MTT experimental results revealed that ESIPT-F is low cytotoxic and has good membrane permeability to cells. Thus, ESIPT-F was further employed to image the tunneling nanotube in vitro HEC-1A cells, displaying high-resolution performance. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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