Interlaboratory comparison for determination of ochratoxin A by ELISA in maize (running title: Determination of ochratoxin A in maize)

Autor: Jakšić Sandra M., Jajić Igor M., Nešić Ksenija D., Stojanov Igor M., Živkov-Baloš Milica M., Mihaljev Željko A., Abramović Biljana F.
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Zdroj: Zbornik Matice Srpske za Prirodne Nauke, Vol 2013, Iss 124, Pp 77-84 (2013)
Druh dokumentu: article
ISSN: 0352-4906
DOI: 10.2298/ZMSPN1324077J
Popis: Participation in interlaboratory comparison and proficiency testing schemes is important for laboratories to control the work quality. In this study, a sample of naturally contaminated maize was analyzed for the content of ochratoxin A (OTA) in three laboratories in Serbia. Participating laboratories used enzymatic immunoaffinity method (ELISA) for the determination of OTA and selection of the ELISA kit was free. Between-laboratory precision was acceptable as evidenced by Cochran’s C test. Moreover, z-scores for all three laboratories were z < ± 2, which is considered acceptable. Used OTA confirmation methods were thin-layer chromatography (TLC) and high-pressure liquid chromatography (HPLC), with fluorescence detector. The results of different methods were comparable. [Projekat Ministarstva nauke Republike Srbije, br. TR 031071 i br. OI 172042]
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