A new in vitro model applied 90Y microspheres to study the effects of low dose beta radiation on colorectal cancer cell line in various oxygenation conditions

Autor:	Piotr Piasecki, Aleksandra Majewska, Jerzy Narloch, Maciej Maciak, Klaudia Brodaczewska, Michal Kuc, Halina Was, Marek Wierzbicki, Krzysztof Brzozowski, Piotr Ziecina, Andrzej Mazurek, Miroslaw Dziuk, Edward Iller, Claudine Kieda
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	Medicine Science
Zdroj:	Scientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
Druh dokumentu:	article
ISSN:	2045-2322
DOI:	10.1038/s41598-021-84000-7
Popis:	Abstract We propose a new in vitro model to assess the impact of 90Y-microspheres derived low-dose beta radiation on colorectal cancer cell line under various oxygenation conditions that mimic the tumor environment. Cancer cells (HCT116) proliferation was assessed using Alamar Blue (AB) assay after 48, 72, and 96 h. FLUKA code assessed changes in cancer cell populations relative to the absorbed dose. In normoxia, mitochondrial activity measured by Alamar Blue after 48–72 h was significantly correlated with the number of microspheres (48 h: r = 0.87 and 72 h: r = 0.89, p
Databáze:	Directory of Open Access Journals
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