DNA methylation and gene expression changes derived from assisted reproductive technologies can be decreased by reproductive fluids

Autor: Sebastian Canovas, Elena Ivanova, Raquel Romar, Soledad García-Martínez, Cristina Soriano-Úbeda, Francisco A García-Vázquez, Heba Saadeh, Simon Andrews, Gavin Kelsey, Pilar Coy
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: eLife, Vol 6 (2017)
Druh dokumentu: article
ISSN: 2050-084X
DOI: 10.7554/eLife.23670
Popis: The number of children born since the origin of Assisted Reproductive Technologies (ART) exceeds 5 million. The majority seem healthy, but a higher frequency of defects has been reported among ART-conceived infants, suggesting an epigenetic cost. We report the first whole-genome DNA methylation datasets from single pig blastocysts showing differences between in vivo and in vitro produced embryos. Blastocysts were produced in vitro either without (C-IVF) or in the presence of natural reproductive fluids (Natur-IVF). Natur-IVF embryos were of higher quality than C-IVF in terms of cell number and hatching ability. RNA-Seq and DNA methylation analyses showed that Natur-IVF embryos have expression and methylation patterns closer to in vivo blastocysts. Genes involved in reprogramming, imprinting and development were affected by culture, with fewer aberrations in Natur-IVF embryos. Methylation analysis detected methylated changes in C-IVF, but not in Natur-IVF, at genes whose methylation could be critical, such as IGF2R and NNAT.
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