Identification and Molecular Characterization of Four New Blastocystis Subtypes Designated ST35-ST38
| Autor: | Jenny G. Maloney, Aleksey Molokin, Raimundo Seguí, Pablo Maravilla, Fernando Martínez-Hernández, Guiehdani Villalobos, Anastasios D. Tsaousis, Eleni Gentekaki, Carla Muñoz-Antolí, Debora R. Klisiowicz, Camila Y. Oishi, Rafael Toledo, J. Guillermo Esteban, Pamela C. Köster, Aida de Lucio, Alejandro Dashti, Begoña Bailo, Rafael Calero-Bernal, David González-Barrio, David Carmena, Mónica Santín |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Microorganisms, Vol 11, Iss 1, p 46 (2022) |
| Druh dokumentu: | article |
| ISSN: | 2076-2607 77550129 |
| DOI: | 10.3390/microorganisms11010046 |
| Popis: | Three recent studies of Blastocystis epidemiology in mammalian hosts identified four novel sequences that appeared to share B. lapemi as the most similar sequence. However, full-length ssu rRNA gene sequences were not available to confirm the validity of these new subtypes. In the present study, Nanopore MinION sequencing was used to obtain full-length reference sequences for each of the new subtypes. Additionally, phylogenetic analyses and pairwise distance comparisons were performed to confirm the validity of each of these new subtypes. We propose that the novel sequences described in this study should be assigned the subtype designations ST35-ST38. The full-length reference sequences of ST35-ST38 will assist in accurate sequence descriptions in future studies of Blastocystis epidemiology and subtype diversity. |
| Databáze: | Directory of Open Access Journals |
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