| Autor: |
Kantima Sangsiriwut, Thanomsak Anekthananon, Winai Ratanasuwan, Wichai Techasathit, Surapol Suwanagool, Surin Khunyok, Teera Kolladarungkri |
| Jazyk: |
angličtina |
| Rok vydání: |
2007 |
| Předmět: |
|
| Zdroj: |
Siriraj Medical Journal, Vol 59, Iss 1 (2007) |
| Druh dokumentu: |
article |
| ISSN: |
2228-8082 |
| Popis: |
Objective: To develop and validate a high-performance liquid chromatography (HPLC) method for the determination of nevirapine in human plasma. Methods: A plasma sample and an internal standard were extracted with tert-butyl methyl ether and determined nevirapine concentration by HPLC method. The limit of quantitation (LOQ), accuracy, precision, specificity, stability and recovery were tested for method validation. Results: Standard curve was linear in the range 0.1 μg/mL to 20 μg/mL. The limit of quantitation was 0.1 μg/ml. Coefficients of variation (CV) of intra-day and inter-day precision were less than 4%. Accuracy was range from 97-101%. The extraction recovery was range from 94-112%. Conclusion: A rapid, sensitive and specific HPLC method was developed and can be used for determination of plasma nevirapine concentration in adult and pediatric patients infected with HIV. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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