Nanopore sequencing reveals methylation changes associated with obesity in circulating cell-free DNA from Göttingen Minipigs
| Autor: | Markus Hodal Drag, Karina Poulsdóttir Debes, Clara Sandkamm Franck, Mette Flethøj, Mille Kronborg Lyhne, Jacob Eifer Møller, Trine Pagh Ludvigsen, Thomas Jespersen, Lisbeth Høier Olsen, Tuomas O. Kilpeläinen |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Epigenetics, Vol 18, Iss 1 (2023) |
| Druh dokumentu: | article |
| ISSN: | 1559-2294 1559-2308 15592294 |
| DOI: | 10.1080/15592294.2023.2199374 |
| Popis: | Profiling of circulating cell-free DNA (cfDNA) by tissue-specific base modifications, such as 5-methylcytosines (5mC), may enable the monitoring of ongoing pathophysiological processes. Nanopore sequencing allows genome-wide 5mC detection in cfDNA without bisulphite conversion. The aims of this study were: i) to find differentially methylated regions (DMRs) of cfDNA associated with obesity in Göttingen minipigs using Nanopore sequencing, ii) to validate a subset of the DMRs using methylation-specific PCR (MSP-PCR), and iii) to compare the cfDNA DMRs with those from whole blood genomic DNA (gDNA). Serum cfDNA and gDNA were obtained from 10 lean and 7 obese Göttingen Minipigs both with experimentally induced myocardial infarction and sequenced using Oxford Nanopore MinION. A total of 1,236 cfDNA DMRs (FDR |
| Databáze: | Directory of Open Access Journals |
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