| Autor: |
Najeeb Halabi, Binitha Thomas, Omar Chidiac, Amal Robay, Julien AbiNahed, Amin Jayyousi, Jassim Al Suwaidi, Martina Bradic, Charbel Abi Khalil |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Cardiovascular Diabetology, Vol 23, Iss 1, Pp 1-23 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
1475-2840 |
| DOI: |
10.1186/s12933-024-02292-1 |
| Popis: |
Abstract Background Monocytes play a central role in the pathophysiology of cardiovascular complications in type 2 diabetes (T2D) patients through different mechanisms. We investigated diabetes-induced changes in lncRNA genes from T2D patients with cardiovascular disease (CVD), long-duration diabetes, and poor glycemic control. Methods We performed paired-end RNA sequencing of monocytes from 37 non-diabetes controls and 120 patients with T2D, of whom 86 had either macro or microvascular disease or both. Monocytes were sorted from peripheral blood using flow cytometry; their RNA was purified and sequenced. Alignments and gene counts were obtained with STAR to reference GRCh38 using Gencode (v41) annotations followed by batch correction with CombatSeq. Differential expression analysis was performed with EdgeR and pathway analysis with IPA software focusing on differentially expressed genes (DEGs) with a p-value |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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