Diagnostic methods for identification of root-knot nematodes species from Brazil
| Autor: | Tiago Garcia da Cunha, Liliane Evangelista Visôtto, Everaldo Antônio Lopes, Claúdio Marcelo Gonçalves Oliveira, Pedro Ivo Vieira Good God |
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| Jazyk: | English<br />Portuguese |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Ciência Rural, Vol 48, Iss 2 (2018) |
| Druh dokumentu: | article |
| ISSN: | 1678-4596 0103-8478 |
| DOI: | 10.1590/0103-8478cr20170449 |
| Popis: | ABSTRACT: The accurate identification of root-knot nematode (RKN) species (Meloidogyne spp.) is essential for implementing management strategies. Methods based on the morphology of adults, isozymes phenotypes and DNA analysis can be used for the diagnosis of RKN. Traditionally, RKN species are identified by the analysis of the perineal patterns and esterase phenotypes. For both procedures, mature females are required. Over the last few decades, accurate and rapid molecular techniques have been validated for RKN diagnosis, including eggs, juveniles and adults as DNA sources. Here, we emphasized the methods used for diagnosis of RKN, including emerging molecular techniques, focusing on the major species reported in Brazil. |
| Databáze: | Directory of Open Access Journals |
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