Methods for glomerular quantification in dogs: a comparative study
| Autor: | Caio Augusto Leles Costa, Charles Silva de Lima, Ricardo Andres Ramirez Uscategui, Gyl Eanes Barros Silva, Leandro Zuccolotto Crivellenti |
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| Jazyk: | English<br />Portuguese |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Ciência Rural, Vol 49, Iss 3 (2019) |
| Druh dokumentu: | article |
| ISSN: | 1678-4596 0103-8478 |
| DOI: | 10.1590/0103-8478cr20180851 |
| Popis: | ABSTRACT: It was evaluated the different methodologies for glomerular enumeration described in the literature in a quantitative, double-blind study on the number of glomeruli present in kidney biopsy specimens obtained using a Tru-cut-type biopsy needle. Eighteen samples were taken (n=18) from various regions of canine kidneys harvested immediately after euthanasia. Sample collection was carried out using Tru-cut-type, semi-automated, 16 gauge needles. Reading and evaluation of the specimens were performed independently by four researchers by means of eye loupe inspection, light microscopy, light microscopy with a reduced condenser aperture, and dissection microscopy. Samples were also subjected to histopathological evaluation for comparison purposes. There was no inter-researcher variation for any of the tested methods. The glomerular counts obtained using light microscopy with reduced condenser aperture were similar to those reported in the histopathological evaluation (P=0.44);whereas, the other glomerular enumeration methods yielded significantly lower counts when compared to the histopathological analysis (eye loupe inspection: P |
| Databáze: | Directory of Open Access Journals |
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