PapG Gene cloning, Escherichia coli uropathogen and examination of its subsequence diversity
| Autor: | Faezeh Hamidiyeh, Mohammad Hassan Shirazi, Jalil Fallah Mehrabadi, Mohammadreza Pourmand, Samaneh Ostad Mohammadi, Hedrousha Molla agha Mirzaei, Davood Afshar |
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| Jazyk: | English<br />Persian |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Iranian South Medical Journal, Vol 16, Iss 1, Pp 1-8 (2013) |
| Druh dokumentu: | article |
| ISSN: | 1735-4374 1735-6954 |
| Popis: | infections are one of the most prevalent human infections. Despite different antigens and toxins of interfering bacteria in infection, one of the important agents in the infections arising from Escherichia coli and the other gram negative bacteria is bacterial binding to host cell surface, so inhibiting the bacterial binding is an appropriate strategy to inhibit the infection. Whereas PapG protein acts as adhesion, it can be an appropriate candidate for developingvaccine. Material and Methods: A Genomic DNA of Escherichia coli bacterium extracted from clinical strain containing PapGII gene. Upon designing primer for PapGII gene, the PCR reaction was applied. The product of PCR was cloned in pBluescript (SK-) plasmid. Using Clustal W and MEGA4 software, the gained subsequence was alignmented with the gene subsequence existing in gene bank and its gene diversity was studied. Results: Based on was down alignment, N terminal on the protein surface and DNA are protected. Conclusion: N terminal domain of PapG gene is a conserved sequence among clinical straines? And it could be used for designing a vaccine against urinary tract infection. |
| Databáze: | Directory of Open Access Journals |
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