Detection of Cronobacter Genus in Powdered Infant Formula by Enzyme-Linked Immunosorbent Assay using Anti-Cronobacter Antibody

Autor: Xinjie Song, Shruti Shukla, Gibaek Lee, Sunhyun Park, Myunghee Kim
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Frontiers in Microbiology, Vol 7 (2016)
Druh dokumentu: article
ISSN: 1664-302X
DOI: 10.3389/fmicb.2016.01124
Popis: Cronobacter species (Cronobacter spp.) are hazardous foodborne pathogens associated with baby food, powdered infant formula (PIF). To develop a rapid and sensitive method for simultaneous detection of seven Cronobacter spp. in PIF, an indirect non-competitive enzyme-linked immunosorbent assay (INC-ELISA) was developed based on a novel immunoglobulin G (IgG), anti-Cronobacter IgG. The developed INC-ELISA was able to detect seven Cronobacter spp. at concentrations ranging from 5.6×103 to 2.1×105 colony forming unit (CFU)/mL in pure culture. Further, INC-ELISA employing anti-Cronobacter IgG was applicable for analysis of PIF samples contaminated with less than
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