Investigation of somatic mutation profiles and tumor evolution of primary oropharyngeal cancer and sequential lymph node metastases using multiregional whole‐exome sequencing

Autor: Nam Suk Sim, Su‐Jin Shin, Inho Park, Sun Och Yoon, Yoon Woo Koh, Se‐Heon Kim, Young Min Park
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Molecular Oncology, Vol 17, Iss 6, Pp 981-992 (2023)
Druh dokumentu: article
ISSN: 1878-0261
1574-7891
DOI: 10.1002/1878-0261.13407
Popis: Lymph node (LN) metastasis is an important factor in determining the treatment and prognosis of oropharyngeal squamous cell carcinoma (OPSCC). Here, we compared the somatic mutational profiles and clonal evolution of primary and metastatic LNs using multiregion sequencing of human papilloma virus (HPV)‐positive OPSCC and HPV‐negative OPSCC. We performed high‐depth whole‐exome sequencing (200×) of 76 samples from 18 patients with OPSCC (10 HPV‐positive and 8 HPV‐negative), including 18 primary tumor samples, 40 metastatic LN samples, and 18 normal tissue samples. Among 40 metastatic LNs, 22 showed extranodal extension (ENE). Mutation profiles of HPV‐positive OPSCC and HPV‐negative OPSCC were similar to those reported previously. Somatic mutations in CDKN2A and TP53 were frequently detected in HPV‐negative OPSCC. Somatic mutations in HPV‐positive OPSCC samples showed APOBEC‐related signatures. Somatic mutations from metastatic LNs showed a different pattern than the primary tumor. Somatic mutations acquired in the WNT pathway during metastasis showed a significant relationship with ENE. Clonal evolution analysis of primary and metastatic LNs showed that, in some cases, each metastatic LN originated from a different primary tumor sub‐clone.
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