A proposed method of sample preparation and homogenization of hemp for the molecular analysis of cannabinoids

Autor: Zachary P. Morehouse, Gabriella L. Ryan, Caleb M. Proctor, Akelachi Okparanta, Will Todd, Derrick Bunting, Taylor White, Steven Parez, Blessida Miller, Veronica Colon, Brandon Easparro, James Atwood, YoungChul Park, Rodney J. Nash
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: SN Applied Sciences, Vol 3, Iss 8, Pp 1-7 (2021)
Druh dokumentu: article
ISSN: 2523-3963
2523-3971
DOI: 10.1007/s42452-021-04747-6
Popis: Abstract The use of Cannabis sativa, or Hemp, in commercial, recreational, and pharmacological applications is on the rise in the United States and worldwide. Many of these applications have guidelines associated with them dependent on the concentration of cannabinoid molecules that keep the products classified as hemp versus marijuana or that allow the producer to comment on the purity and potency of their product. Herein, we propose a method for homogenization of hemp that results in small particle sizes, uniform samples, and does not alter the cannabinoid concentrations during processing, allowing for optimal and reproducible potency testing. Using a novel “active grinding media” we homogenized commercially available hemp to analyze approximately 100 mg samples of homogenate via sieve analysis and high-performance liquid chromatography to assess the resulting size and potency of the sample when using this methodology. When processing hemp samples with our proposed methodology, we have demonstrated the ability to produce 60.2% of all particles
Databáze: Directory of Open Access Journals
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