Detection and Quantization of the Expression of Two mu-Opioid Receptor Splice Variants mRNA (hMOR-1A and hMOR-1O) in Peripheral Blood Lymphocytes of Long-Term Abstinent Former Opioid Addicts
| Autor: | M.R. Zarrindast, T. Sedaghati, A. Goodarzi, F. Roushanzamir, N. Vousooghi, Noori Daloii |
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| Jazyk: | perština |
| Rok vydání: | 2008 |
| Předmět: | |
| Zdroj: | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum, Vol 2, Iss 2, Pp 9-14 (2008) |
| Druh dokumentu: | article |
| ISSN: | 1735-7799 2008-1375 |
| Popis: | Background and ObjectivesThe mu-Opioid receptor (MOR) exerts a critical role on effects of opiodis. The objective of this study is to find a peripheral bio-marker in addiction studies through quantization of the expression of two MOR splice variants mRNA (hMOR-1A and hMOR-1O) in peripheral blood lymphocytes (PBLs) of long-term abstinent former opioids addicts.MethodsIn this case-control study, case and control people were male and divided in two groups: people who gave up addiction to opioids (case) and healthy individuals without history of addiction (control). The mRNA expression in PBLs of participants was detected and measured by real-time Polymerase Chain Reaction (PCR) using SYBR Green Dye.ResultsThe hMOR-1A mRNA expression in PBLs of abstinent group was significantly reduced and reached to 0.33 of the control group (p |
| Databáze: | Directory of Open Access Journals |
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