Kinetics of TNF-alpha and IFN-gamma mRNA expression in islets and spleen of NOD mice
| Autor: | D. Ventura-Oliveira, C.A. Vilella, M.E. Zanin, G.M. Castro, D.C. Moreira Filho, R.L. Zollner |
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| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: | |
| Zdroj: | Brazilian Journal of Medical and Biological Research, Vol 35, Iss 11, Pp 1347-1355 (2002) |
| Druh dokumentu: | article |
| ISSN: | 0100-879X 1414-431X |
| DOI: | 10.1590/S0100-879X2002001100013 |
| Popis: | Insulin-dependent diabetes mellitus is caused by autoimmune destruction of pancreatic ß cells. Non-obese diabetic (NOD) mice spontaneously develop diabetes similar to the human disease. Cytokines produced by islet-infiltrating mononuclear cells may be directly cytotoxic and can be involved in islet destruction coordinated by CD4+ and CD8+ cells. We utilized a semiquantitative RT-PCR assay to analyze in vitro the mRNA expression of TNF-alpha and IFN-gamma cytokine genes in isolated islets (N = 100) and spleen cells (5 x 10(5) cells) from female NOD mice during the development of diabetes and from female CBA-j mice as a related control strain that does not develop diabetes. Cytokine mRNAs were measured at 2, 4, 8, 14 and 28 weeks of age from the onset of insulitis to the development of overt diabetes. An increase in IFN-gamma expression in islets was observed for females aged 28 weeks (149 ± 29 arbitrary units (AU), P |
| Databáze: | Directory of Open Access Journals |
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