Trypsin Inhibitor Isolated From Glycine max (Soya Bean) Extraction, Purification, and Characterization

Autor: Sabir Khan, Shafia Arshad, Amina Arif, Rida Tanveer, Zeemal Seemab Amin, Saba Abbas, Amna Maqsood, Muhammad Raza, Arooj Munir, Amara Latif, Maryam Habiba, Muhammad Afzal
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Dose-Response, Vol 20 (2022)
Druh dokumentu: article
ISSN: 1559-3258
15593258
DOI: 10.1177/15593258221131462
Popis: The current study aims to isolate, purify, and characterize the trypsin inhibitor protein from seeds of soya beans, scientifically known as Glycine max . Its seeds were ground, and the powder was soaked several times using n-hexane. It was added to phosphate buffer saline (PBS) followed by filtration and centrifugation of the PBS dissolved extract. The supernatant was subjected to ammonium sulfate precipitation and about six fractions, 30% to 80% were prepared. The centrifuged pellets obtained from each fraction were dialyzed and run on SDS-PAGE. The trypsin inhibitor protein was precipitated and characterized in 30% pellet and molecular weight was 21.5 kDa compared to protein ladder (ThermoFisher 10-170 kDa). GC-MS analysis revealed the steroid derivatives such as stigmasterol, campesterol, beta-sitosterol, and gamma-tocopherol. Glycine max trypsin inhibitor could be used as a plant-derived drug to overcome the over-activation of trypsin without its real substrate (proteins) becoming activated and start auto digestion leading to pancreatitis.
Databáze: Directory of Open Access Journals
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje