| Autor: |
Yunfeng Cui, Yangyang Ji, Xi Chen, Jianjiong Li, Jinhui Feng, Qing Zhao, Peiyuan Yao, Qiaqing Wu, Dunming Zhu |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Green Synthesis and Catalysis, Vol 4, Iss 3, Pp 268-271 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2666-5549 |
| DOI: |
10.1016/j.gresc.2022.06.010 |
| Popis: |
(S)-1-(3′-Bromo-2′-methoxyphenyl)ethanol ((S)-1b) is the key precursor for the synthesis of Lusutrombopag. The bioreduction of 1-(3′-bromo-2′-methoxyphenyl)ethanone (1a) offers an attractive method to access this important compound. Through screening the available carbonyl reductases, we obtained a carbonyl reductase from Novosphingobium aromaticivorans (CBR), which could completely convert 100 g/L of 1a to (S)-1b. Furthermore, a carbonyl reductase from Novosphingobium sp. Leaf2 (NoCR) was identified to completely convert 200 g/L of 1a to (S)-1b with excellent enantioselectivity (>99% ee) and 77% isolated yield using FDH/formate system for NADH regeneration. The Km and kcat of recombinant NoCR towards 1a were 0.66 mmol/L and 7.5 s-1, and the catalytic efficiency kcat/Km was 11.3 mmol/s.L. Meanwhile, NoCR showed high catalytic activity and stereoselectivity towards acetophenone derivatives with halogen or methoxy substitution on the benzene ring, indicating that NoCR is a valuable biocatalyst with potential practical applications. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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