Quantum dots based in-vitro co-culture cancer model for identification of rare cancer cell heterogeneity

Autor: Satyanarayana Swamy Vyshnava, Gayathri Pandluru, Kanderi Dileep Kumar, Shiva Prasad Panjala, Swathi Banapuram, Kameshpandian Paramasivam, Kothamunireddy Varalakshmi Devi, Roja Rani Anupalli, Muralidhara Rao Dowlatabad
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Scientific Reports, Vol 12, Iss 1, Pp 1-12 (2022)
Druh dokumentu: article
ISSN: 2045-2322
DOI: 10.1038/s41598-022-09702-y
Popis: Abstract Cancer cell heterogeneity (CCH) is crucial in understanding cancer progression and metastasis. The CCH is one of the stumbling blocks in modern medicine's therapeutics and diagnostics . An in-vitro model of co-culture systems of MCF-7, HeLa, HEK-293, with THP-1 cells showed the occurrence of EpCAM positive (EpCAM+) and EpCAM negative (EpCAM−) heterogenetic cancer cell types labeled with the Quantum Dot antibody conjugates (QDAb). This in-vitro model study could provide insights into the role of rare cancer cells manifestation and their heterogeneity in metastatic progression and risk for severe infections in these patients. We successfully report the presence of CCH based on the fluorescence ratios of the co-cultured cancer cells when treated with the QDAb. These short-term mimic co-cultures give a compelling and quite associated model for assessing early treatment responses in various cancers.
Databáze: Directory of Open Access Journals
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