PCR-RELP for detecting of Theileria annulata infection in cattle and Hyalomma species in Kermanshah Province, Iran
| Autor: | Ghashgai, O., Yakhchali, M., Sohrabi, S. |
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| Jazyk: | English<br />French |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Archives of Razi Institute, Vol 70, Iss 01, Pp 7-12 (2015) |
| Druh dokumentu: | article |
| ISSN: | 0365-3439 2008-9872 |
| Popis: | Bovine theileriosis is important disease in tropical and subtropical areas with great economic losses in livestock husbandry in Iran. The aim of study was to assess the prevalence of Theileria annulata infection in cattle and Hyalomma species of Kermanshah Province, Iran. A number of 138 blood samples were randomly taken from examined cattle. The genomic DNA was extracted and PCR was performed to specifically amplify a 721-bp-long fragment of the 30 Kilo Dalton major merozoite surface antigen (30 KDa msa) of T. annulata. The amplified products were digested with TaqI, Rasl, and AluI restriction enzymes. Overall prevalence was 9.44% (13/138) with lymphadnopathy (1.17%) and pale mucosal membrane (1.9%) in Holstein cattle aged |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
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