| Autor: |
Seokju Seo, Ramya Ganiga Prabhakar, Saoirse Disney-McKeethen, Xinhao Song, Yousif Shamoo |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
STAR Protocols, Vol 3, Iss 2, Pp 101332- (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1667 |
| DOI: |
10.1016/j.xpro.2022.101332 |
| Popis: |
Summary: Microdroplet emulsions allow investigators to build controllable microenvironments for applications in experimental evolution and synthetic ecology. We designed a microfluidic platform that uses highly homogenous microdroplets to enable these experiments. We also present a step-by-step protocol for the rapid production of highly homogeneous microdroplets suitable for experimental evolution. We also describe protocols for the propagation and serial passage of microbial populations across a range of selection schemes and potential spatial structures.For complete details on the use and execution of this protocol, please refer to Seo et al. (2021). |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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