Autor: |
WANG Xiu-mei, WANG Kang-kang, CHEN Xiao-yan, LUO Jin-jian |
Jazyk: |
čínština |
Rok vydání: |
2020 |
Předmět: |
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Zdroj: |
Jichu yixue yu linchuang, Vol 40, Iss 7, Pp 955-959 (2020) |
Druh dokumentu: |
article |
ISSN: |
1001-6325 |
Popis: |
Objective To investigate whether dihydrotanshinone Ⅰ could regulate the biological activities of proliferation, migration and apoptosis of esophageal squamous cell carcinoma cell line EC9706 through Wnt/β-catenin signaling pathway. Methods EC9706 cells were cultured in medium containing 0, 10, and 30 μmol/L dihydrotanshinoneⅠ solution, and cell proliferation was examined with a cell counting kit-8, cell migration was checked with scratch test. Western blot was used to detect β-catenin, cyclin D1 and c-myc protein expression in cells. Apoptosis was detected by flow cytometry. Results DihydrotanshinoneⅠ effectively inhibited the proliferation and migration of EC9706 cells. DihydrotanshinoneⅠ down-regulated β-catenin, cyclin D1 and c-myc protein expression in cells (P<0.05). DihydrotanshinoneⅠ promoted apoptosis in a dose-dependent manner. Conclusions DihydrotanshinoneⅠ can inhibit proliferation and migration of EC9706 cells and promote the cells apoptosis. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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